The next year, PacBio developed a new method to allow multiple sequencing passes on the same DNA molecule. Double stranded DNA templates were ligated to two single stranded adapters, creating what the company called a “SMRTbell template.” Sequencing began at a primer on one of the adaptors and could proceed multiple times per molecule due to circularization, in a process called rolling-circle amplification. This helped reduce PacBio’s error rates significantly.
哈梅內伊：統治伊朗37年的最高領袖是誰？
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“制定和实施五年规划是我们党治国理政一条重要经验，是中国特色社会主义制度一个重要政治优势”，在“开年第一课”上，总书记强调：“党的二十届四中全会对‘十五五’时期经济社会发展作出战略部署，要全面深刻准确领会和把握。”